| Registro completo |
| Provedor de dados: |
ArchiMer
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| País: |
France
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| Título: |
Characterization of molecular processes involved in the pearl formation in Pinctada margaritifera for a sustainable development of pearl farming industry in French Polynesia
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| Autores: |
Gueguen, Yannick
Montagnani, Caroline
Joubert, Caroline
Marie, Benjamin
Belliard, Corinne
Tayale, Alexandre
Fievet, Julie
Levy, Peva
Piquemal, David
Marin, Frederic
Le Moullac, Gilles
Ky, Chin-long
Garen, Pierre
Lo, Cedrick
Saulnier, Denis
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| Data: |
2013
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| Ano: |
2013
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| Palavras-chave: |
Pearl oyster
Biomineralization
Pinctada margaritifera
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| Resumo: |
Tahiti’s pearl farming industry plays a major socio-economic role in French Polynesia. In an increasingly competitive market where the production of high quality pearls becomes essential, research can help secure and ensure sustainable production. In that context, Ifremer, in close collaboration with the “direction des resources marines” (French Polynesian government agency) has developed research projects on the “sustainable development of pearl farming”. This program is organized along 3 axes: (1) understanding the animal physiology and initiating a genetically selective breeding program of donor oysters; (2) understanding pearl oyster larvae dispersal and recruitment; (3) understanding the molecular mechanisms underlying biomineralization processes during shell and pearl formation. It is in this frame that we have developed a highthroughput Expressed Sequence Tags pyrosequencing program on the calcifying mantle, combined with proteomic analyses of the shell and pearl. We analyzed 276738 EST sequences, leading to the constitution of a P. margaritifera mantle transcripts catalogue of 82 sequences potentially involved in biomineralization. Our results provided direct evidence that our ESTs data set covers a large number of the matrix proteins of P. margaritifera. In addition, our proteomic analysis enabled us to retrieve, in silico, all the sequences from P. margaritifera involved in the biomineralization process already published on databases. Integration of these two methods allowed, for the first time, the global composition of calcifying tissue and calcified structures to be examined in tandem.
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| Tipo: |
Text
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| Idioma: |
Inglês
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| Identificador: |
http://archimer.ifremer.fr/doc/00167/27840/26049.pdf
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| Relação: |
http://archimer.ifremer.fr/doc/00167/27840/
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| Formato: |
application/pdf
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| Fonte: |
Recent Advances in Pearl Research—Proceedings of the International Symposium on Pearl Research 2011.Edited by Shugo Watabe, Kaoru Maeyama and Hiromichi Nagasawa. 2013. ISBN : 978-4-88704-158-5. pp.184-194
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| Direitos: |
2013 TERRAPUB. All rights reserved.
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